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human reference cdna library  (TaKaRa)


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    TaKaRa human reference cdna library
    Human Reference Cdna Library, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+reference+cdna+library/qPCR+Human+Reference+cDNA%2C+Random-primed/pmc12210326-426-12-16
    Average 93 stars, based on 47 article reviews
    human reference cdna library - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Sequencing:

    Article Title: Mapping the developmental trajectory of human astrocytes reveals divergence in glioblastoma
    Article Snippet: .. The ORF sequence for Ascl1 ( NM_004316 ) was amplified from the human reference cDNA library (Takara 639654) and cloned into the multiple cloning sites of pCW57-GFP-2A-MCS (Addgene #71783) using AvrII and BamHI restriction sites. .. These transfer plasmids were then transfected into HEK298FT cells with Lipofectamine 3000 (Invitrogen - L3000–015) to produce lentivirus following manufacturer’s protocols.

    Amplification:

    Article Title: Mapping the developmental trajectory of human astrocytes reveals divergence in glioblastoma
    Article Snippet: .. The ORF sequence for Ascl1 ( NM_004316 ) was amplified from the human reference cDNA library (Takara 639654) and cloned into the multiple cloning sites of pCW57-GFP-2A-MCS (Addgene #71783) using AvrII and BamHI restriction sites. .. These transfer plasmids were then transfected into HEK298FT cells with Lipofectamine 3000 (Invitrogen - L3000–015) to produce lentivirus following manufacturer’s protocols.

    cDNA Library Assay:

    Article Title: Mapping the developmental trajectory of human astrocytes reveals divergence in glioblastoma
    Article Snippet: .. The ORF sequence for Ascl1 ( NM_004316 ) was amplified from the human reference cDNA library (Takara 639654) and cloned into the multiple cloning sites of pCW57-GFP-2A-MCS (Addgene #71783) using AvrII and BamHI restriction sites. .. These transfer plasmids were then transfected into HEK298FT cells with Lipofectamine 3000 (Invitrogen - L3000–015) to produce lentivirus following manufacturer’s protocols.

    Clone Assay:

    Article Title: Mapping the developmental trajectory of human astrocytes reveals divergence in glioblastoma
    Article Snippet: .. The ORF sequence for Ascl1 ( NM_004316 ) was amplified from the human reference cDNA library (Takara 639654) and cloned into the multiple cloning sites of pCW57-GFP-2A-MCS (Addgene #71783) using AvrII and BamHI restriction sites. .. These transfer plasmids were then transfected into HEK298FT cells with Lipofectamine 3000 (Invitrogen - L3000–015) to produce lentivirus following manufacturer’s protocols.

    Cloning:

    Article Title: Mapping the developmental trajectory of human astrocytes reveals divergence in glioblastoma
    Article Snippet: .. The ORF sequence for Ascl1 ( NM_004316 ) was amplified from the human reference cDNA library (Takara 639654) and cloned into the multiple cloning sites of pCW57-GFP-2A-MCS (Addgene #71783) using AvrII and BamHI restriction sites. .. These transfer plasmids were then transfected into HEK298FT cells with Lipofectamine 3000 (Invitrogen - L3000–015) to produce lentivirus following manufacturer’s protocols.



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